The Bioscan, 3(4): 463-468, 2008
An International Quarterly Journal of Life Sciences
web site: www.thebioscan.in 
VALIDATED HPTLC METHOD FOR QUANTITATIVE ANALYSIS OF 
ESOMEPRAZOLE IN TABLETS
 
S. B. CHEPURWAR*, S. A. POLSHETTIWAR1, M.S. WANI1 AND V. N. JAGTAP2
Emcure Pharmaceutical Ltd., Plot No.2, I.T.B.T. Park, Hinjewadi, Pune - 411 057 
1MAEER’S, Maharashtra Institute of Pharmacy, M.I.T. Campus, Kothrud, Pune - 411 038, INDIA
2MCE Society, Institute of Pharmacy, Azam Campus, Pune - 441 001
E-mail: contact_psatish@yahoo.co.in 

KEY WORDS
Esomeprazole
magnesium trihydrate
HPTLC
Densitometric analysis


Received on: 21.05.2008


Accepted on: 19.10.2008



*Corresponding
 Author
 

ABSTRACT

A simple, rapid, and reliable High performance thin layer chromatography (HPTLC) method has been established for determination of Esomeprazole (ES) in tablets. Identification and determination were performed on aluminum backed silica gel 60 F254 TLC plates, previously washed with methanol. The mobile phase was Ethyl acetate: ammonia, 8 + 0.8 (v/v). This system was found to give compact spots for esomeprazole (Rf = 0.60 ± 0.01). Densitometric analysis of esomeprazole was carried out in the absorbance mode at 301 nm.The linear regression data for the calibration plots should good linear relationship with r2 = 0.9992 in the concentration range 100 – 500 ng/spot. The suitability of this HPTLC method for quantitative determination of compound was proved by validation in accordance with the requirements of ICH guidelines. The method was used for determination of the compound in commercial pharmaceutical dosage forms. The method is simple, reproducible, and accurate and is a more effective option than other chromatographic techniques in routine quality control.